Page 262 - PC2019 Program & Proceedings

PLANT CANADA 2019

P97. Evaluation of tissue culture and cloning propagation efficiencies of three industrial hemp
varieties
El-Mezawy, A.; J. Slaski
(InnoTech Alberta)

Tissue culture and vegetative cloning techniques are used by hemp producers to propagate and preserve
plant genetics. We evaluated the efficiency of both techniques on three hemp varieties, Katani, Silesia and
X59. For tissue culture propagation, five seed surface sterilization methods were compared. Sterile seeds
were grown on ½ MS media for 8 days at 24 °C for 16 h in light and 22 °C for 8 h in dark. Hypocotyl,
leaf segment and cotyledon were cut and plated on MS callus-induction media with 6.79 µM 2,4-
Dichlorophenoxyacetic acid. Explants were monitored and transferred to fresh MS-shoot induction media
then root elongation media based on their development. Rooted plants were transferred to pots with
potting soil mix and grown to maturity. For cloning propagation, varieties were grown directly from seed
in Promix potting soil. Terminal buds were removed from female plants at the 6-8 leaf stage. Lateral
branches were excessed, treated with rooting hormone and planted in training pots for root establishment.
Clones with established roots were transferred to a 7-inch pots and grown to maturity. The seed-born
fungal contamination in hemp seed and the difficulty for shoots to develop roots in rooting media were
the main obstacles for tissue culture propagation. Vegetative cloning was more efficient in producing
numerous clones identical to the mother plant compared to tissue culture techniques.

Aliaa El-Mezawy (aliaa.el-mezawy@innotechalberta.ca)

P98. Characterization of nested association mapping population in dry bean
*
Vazin, M. ; T. Smith; K.P. Pauls
Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada

The common bean (Phaseolus vulgaris L.) is grown widely around the globe, providing an important
staple food crop for subsistence farmers in the developing countries and a high-value commodity crop in
the developed world. Understanding the genetic bases of different agronomic traits and quality traits are
important for efforts to breed improved lines in common bean. The Nested Association Mapping (NAM)
population of F4:5 recombinant inbred lines (RILs) was created with the cultivar Ex Rico 23, and 10
founder lines that span the genetic diversity of Ontario Mesoamerican germplasm. The NAM population
was evaluated for different agronomic traits including yield, days to 50% flowering, and days to maturity
in the field in four environments in 2016 and 2017 at the Elora Research Station (ERS) and Woodstock
Research Station (WRS), and will be genotyped using Genotyping by Sequencing (GBS).

The distribution of all the traits, days to 50% flowering, days to maturity, and yield was continuous and
showed some transgressive segregation compared to the parental lines for each environments. Statistical
analysis of all four environments showed highly significant phenotypic effects for all the traits measured
(P=0.05).

Maryam Vazin (vazinm@uoguelph.ca)

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