Page 261 - PC2019 Program & Proceedings

PLANT CANADA 2019

P95. Genetic transformation of oat to elucidate a gene associated with beta-glucan
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Fatmawati, A. ; M. Mahmoud; T. Donoso; W. Chen ; R. Kaur; N. Tinker ; J. Singh
1 McGill University
2
Agriculture and Agri-food Canada
Oat is known to reduce blood pressure and cholesterol. This has been attributed to its high β-glucan
content. β-glucan is a major non-starch component in oat, consisting of a double β-1,3 and β-1,4 linkages.
Recently, a Thaumatin Like Protein, TLP8 has been identified in barley which interacts with β-glucan and
regulates its content in the grain. Higher transcript abundance of TLP8 in barley grains reflects a lower
amount of β-glucan and vice-versa. In the current study, we hypothesized that the downregulation of
TLP8 could increase β-glucan content in oat. The TLP8 homolog in oat was retrieved and the RNAi
construct has been created for oat transformation. The genetic transformation was conducted via
bombardment gun method. Transformants were generated successfully in oat variety “Park”, using PAT
gene for selection, yielding a 17.2% transformation efficiency. Histochemical assay confirmed the
expression of PAT gene and oat transgenic plants were found to be resistant to herbicide LIBERTY
(0.2%) exposure. Currently, we are conducting a molecular characterization of transgenic lines in order
to explain the association between TLP8 and β-glucan in oat.

Annis Fatmawati (annis.fatmawati@mail.mcgill.ca)

P96. Identification of five QTLs for clubroot resistance to three novel pathotypes of
Plasmodiophora brassicae in Brassica oleracea through genotyping-by-sequencing
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Karim, M. ; F. Fuyou ; A. Dakouri ; S. Strelkov ; B. Gossen ; G. Peng ; F. Yu
1
1 Agriculture and Agri-Food Canada
University of Alberta
2

Managing clubroot disease of canola in the Canadian prairies is a challenging problem due to the
development of new pathotypes. Clubroot disease in canola widely managed with major genes, which is
effective against single pathotypes, but multigenic resistance is effective against multiple pathotypes. B.
rapa and B. oleracea are considered major sources of qualitative and quantitative resistance of clubroot
disease, respectively. In this study, genotyping-by-sequencing (GBS) method was used to construct a
high-density genetic map and to identify QTLs for clubroot resistance to three novel pathotypes 2B, 3A
and 3D in backcross population derived from the cross between the resistant cabbage variety ‘Badger
Shipper’and the susceptible double haploid line TO10000DH3. A total of 42,923 SNPs were identified
from 92 BC1 lines of which 10,289 SNPs found polymorphic. A total of 914 cM genetic map was
constructed by 1,842 high quality polymorphic SNPs. Two QTLs, CRQTL_2B_1 and CRQTL_2B_2 were
detected for resistance to pathotype 2B on chromosome C1 and C3 with phenotypic variation explained
(PEV) of 15.9 and 15.5%. A single QTL, CRQTL_3A_1 was detected for resistance to pathotype 3A on
chromosome C3 with PEV of 19.3%. Two QTLs, CRQTL_3D_1 and CRQTL_3D_2 were detected for
resistance to pathotype 3D on chromosome C3 and C4 with PEV of 11.6 and 13.5%. Associated SNP
markers can be used for marker assisted breeding for clubroot resistance in canola

Md. Masud Karim (masud.karim@canada.ca)

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