Page 257 - PC2019 Program & Proceedings

PLANT CANADA 2019

P87. Leaf rust resistance genes in Canadian wheat cultivars Red Fife, Stettler, Vesper, Lillian,
Carberry and AC Cadillac
1
2
McCallum, B. ; F. Bokore ; R. Cuthbert ; R. Knox 2
2
1 Agriculture and Agri-Food Canada
2 AAFC
The wheat cultivars Stettler, Vesper, Lillian, Carberry and AC Cadillac are widely grown in Canada and
have been frequently used as parents for the next generation of Canadian wheat cultivars. It is important
to understand the genetic basis of leaf rust (Puccinia triticina Eriks.) resistance in these cultivars to
improve this resistance in future cultivars. Five doubled haploid populations were analyzed for leaf rust
resistance both in the field and using individual isolates indoors; Stettler/Red Fife, Vesper/Lillian,
Vesper/Stettler, Carberry/Vesper, and Carberry/AC Cadillac. Preliminary gene determinations were
made on these lines based on segregation of genes at the seedling stage, molecular markers, and QTLs
determined by analysis of field data. Red Fife had two seedling resistance genes, that appear to be the
temporarily named genes LrMar and LrCen. Stettler was thought to have Lr2a and Lr16, Vesper was
hypothesized to have Lr21 and Lr17. Lillian had a seedling resistance gene on chromosome 4A that
could not be identified, Lr34 and LrCen. Carberry had Lr2a, Lr16 and Lr34 and AC Cadillac had Lr10
and Lr34.

Brent McCallum (brent.mccallum@canada.ca)

P88. Genetic analysis and molecular mapping of the oat crown rust seedling resistance gene Pc39
2
1
1
3
Zhao, J. ; A. Kebede ; Menzies, J. ; N. Tinker ; C. McCartney
1
1 Morden Research and Development Centre
2 Agriculture and Agri-Food Canada
3 Ottawa Research and Development Centre
Crown rust is a major disease of oat with race-specific seedling resistance genes being a primary means
for control. Pc39 is a seedling crown rust resistance gene that has been used in oat breeding programs.
The objective of this study was to develop DNA markers linked to Pc39 for marker-assisted selection.
Two RIL populations developed from the crosses AC Assiniboia/MN841801 and AC
Medallion/MN841801 were phenotyped at the seedling stage using oat crown rust race LMBG. Pc39 was
mapped to a linkage group consisting of 16 SNP markers, which placed the gene on Mrg11 LG of the oat
consensus map. Pc39 co-segregated with marker GMI_ES01_c12570_390, and was flanked by two
markers avgbs_126086.1.41 and GMI_ES15_c276_702 with genetic distances of 1.7 cM and 0.3 cM in
AsbMN. Similar results were obtained in MedMN with genetic distances of 0.4 cM and 0.4 cM. SNPs
were converted to KASP markers. Two SNP loci defined a haplotype that predicted the presence of Pc39
in a panel of oat germplasm. The newly developed markers could find applications in the identification of
the Pc39 gene in oat germplasm and the positional cloning of this gene.

Jim Menzies (jim.menzies@canada.ca)

Page 255 of 339