Page 177 - PC2019 Program & Proceedings

PLANT CANADA 2019

S139. Genetic diversity in public soybean breeding programs
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Bruce, R. ; D. Torkamaneh ; A. Ficht ; C. Grainger ; F. Belzile ; M. Eskandari ; I. Rajcan 1
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1 University of Guelph
2 Université Laval
Studying crop breeding programs can provide important insight into the mechanisms of crop
improvement from a historical context and identify strategies for future crop improvement. To study
diversity in soybean breeding germplasm a panel of 296 pedigree-related accessions was characterized,
representing decades of breeding in two programs at the University of Guelph – the Guelph Campus
(maturity groups [MG] 0 and I) and the Ridgetown Campus (MG II). Field trials were carried out in
multiple environments in Ontario to measure agronomic and seed composition traits. Within the soybean
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cultivars, significant yield increases of 17.1 kg⸱ha ⸱year and 15.7 kg⸱ha ⸱year were identified in
Guelph and Ridgetown Campus programs, respectively. Protein and 100-seed weight were also
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significantly increasing in Ridgetown cultivars (0.76 g⸱kg year and 0.1 g⸱year ). Genotyping the
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accessions resulted in 76,549 SNPs. Structure analysis did not identify stratification between breeding
program and historical accessions. Nucleotide diversity analysis revealed that historical accessions were
the most diverse, however, breeding has significantly increased genetic diversity in recent years. Genome-
wide association identified regions significantly associated with seed and agronomic traits and further
haplotype analysis has uncovered trends in the genomic regions associated these traits. Comparison of the
University of Guelph’s breeding germplasm to the germplasm accessions in the USDA gene bank showed
that only a portion of the available genetic diversity in soybean has been used for breeding at Guelph.
Robert Bruce (rbruce@uoguelph.ca)

S140. Identification of a potential candidate gene for the E8 maturity locus in soybean (Glycine
max)
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Sadowski, M. ; B. Samanfar ; E. Cober ; M. Charette ; F. Dehne ; J. Green ; A. Golshani 1
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1 Carleton University; Agriculture and Agri-Food Canada; AAFC Ottawa-RDC
Soybean is one of the largest sources of vegetable oil and protein in the world, and also an important
legume crop to the Canadian economy. In order to expand soybean further north and west in Canada, the
identification and characterization of genes involved in time of flowering and maturity are crucial. The E8
maturity locus was previously identified in our lab using classical breeding practices and a genome-wide
SSR marker analysis, revealing a large, high confidence region. This region has been investigated by a
functional genomics approach using a bioinformatics tool called Soybean-PIPE (Protein-Protein
Interaction Prediction Engine) which predicts genome wide protein-protein interactions. To filter the
~1000 genes of the E8 region, each gene was ordered based on how many of its interacting partners
(genes) were involved in development and flowering, shortlisting the candidate region down to ~25
genes. A likely candidate Glyma.04G124300, is annotated with FAR-RED ELONGATED
HYPOCOTYLS 3 (FHY3) and FAR-RED-IMPAIRED RESPONSE 1 (FAR1) functions. These proteins
are known to have multifaceted roles in light signaling and various physiological and developmental
processes. Sequencing contrasting lines for E8 and e8 revealed a number of single nucleotide
polymorphisms (SNPs) and insertion and deletion (INDELs), altogether suggesting that
Glyma.04G124300 is the likely candidate gene for maturity locus E8.

Michael Sadowski (mike.sadowski@outlook.com)

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