Page 173 - PC2019 Program & Proceedings

PLANT CANADA 2019

S131. Optimizing growing conditions for romaine lettuce (Lactuca Sativa L. var. Longifolia)
production in a plant factory
*
Bayley, D. ; T. Graham; M. Dixon
University of Guelph

Plant Factories (PF) are indoor crop production facilities utilizing high cropping densities and vertically
integrated growing layers. These systems generally require less inputs (water, fertilizer, pesticides, etc.)
compared to traditional farming and take advantage of volume use efficiencies via vertical integration. In
order to realize the full potential of these production systems the correct complement of environmental
conditions (e.g., light, CO2, humidity, nutrition, etc.) needs to be developed for each crop. There is ample
research supporting the production of leaf lettuce cultivation in these facilities; however, there is no such
body of information for romaine lettuce, a popular and economically significant type of lettuce. The
presented study examines the response of romaine lettuce to variable planting densities, and its
hydroponic nutrient requirements for PF production.

Daniel Bayley (dbayley@alumni.uoguelph.ca)

S132. Monitoring of functional state of in vitro preserved plants in Lavandula angustifolia Mill.
Brailko, V. ; I. Mitrofanova ; N. Ivanova ; O. Mitrofanova ; I. Novikov 3
1
2
1
1
1 FSFIS "The Nikita Botanical Gardens – National Scientific Center of the RAS, Yalta
2 FSFIS "The Nikita Botanical Gardens - National Scientific Center of the RAS"
3 Research Institute of Agriculture of Crimea

The resistance of chlorophyll-containing tissues to excess light is widely used in experimental biology as
an integral criterion for the functional state of plants. The parameters of fluorescence induction of
microshoots in lavender plants at different temperatures (4 to 12°C), duration of conservation and light
-2 -
intensity 1.25-3.75 μM m s 1 in genebank in vitro were investigated. The conservation was carried out
on ¼ MS medium with sucrose and chlorocholin chloride. After 6 months of depositing, the indicator (Fm
- Fst) / Fm in cultivars ‘Record’ and ‘Belyanka’ approached the suboptimal values (0.38 ... 0.53 a.u.). At
this time photosystem of the plants not damaged and an active production processes are suspended. The
temperature significantly affects the functional state of microshoots: optimal temperatures of 4°C and 6°C
after 6 months and 12 months of conservation have been identified. Temperature going up was induced
2
the increasing of (Fm - Fst) / Fm indicator (r = 0.23 ... 0.43; R = 0.03 ... 0.68). This study was funded by
the ST No. 0829-2019-0038 of the FSFIS “NBG-NSC”.

Valentina Brailko (valentina.brailko@yandex.ru)

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