Page 197 - Plant Canada 2024 Proceeding

PLANT CANADA 2024

Pt nor to the Triticum aestivum genomes, but which match short open reading frames on the Pt genome.
The most recent research progress will be presented.

*[O185] CHANGES IN SENSITIVITY OF CLARIREEDIA JACKSONII TO THE DEMETHYLATION
INHIBITOR FUNGICIDE PROPICONAZOLE AFTER 30 YEARS OF USE. Andrea Rether , Mikaela
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Ryan , Nava Brimble , Alexa Nguyen , and Tom Hsiang . School of Environmental Sciences, University
1
of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1
Correspondence to: arether@uoguelph.ca

Dollar spot is the most prevalent turfgrass disease in the Great Lakes Region, and repeat fungicide use is
required to prevent aesthetic damage caused by symptoms. Repeat fungicide applications of the same
chemical family select for fungicide-resistant biotypes in fungal populations. There are reports of
decreased sensitivity to propiconazole, a demethylation inhibiting (DMI) fungicide, in populations of
Clarireedia jacksonii, the causal agent of dollar spot. In 1993, a baseline study was conducted where
eight populations of C. jacksonii in Ontario were sampled and tested for sensitivity to propiconazole. This
study was repeated 10 and 20 years later where six of the original eight populations were sampled. In
2023, twelve populations of C. jacksonii in Ontario were sampled and tested for sensitivity to
propiconazole. EC50 values (effective concentration for 50% inhibition of growth) were generated for all
981 isolates collected. Isolates collected in this study were less sensitive (mean EC50 = 0.079 μg/ml) than
those collected in 2013 (mean EC50 = 0.054 μg/ml), 2003 (mean EC50 = 0.026 μg/ml), and the baseline
study (mean EC50 = 0.008 μg/ml). Future work assessing the relationship between mean EC50 values and
the number of DMI applications made on the sampled dollar spot populations will increase our
understanding of field resistance risk in Ontario.

*[O186] IMPROVING BACTERIAL LEAF STREAK MANAGEMENT IN WHEAT: DEVELOPMENT OF A
RAPID LOOP-MEDIATED AMPLIFIATION (LAMP) PROTOCOL FOR SEED TESTING. Valentina
Anastasini , Heting Fu , Jie Feng , T. Kelly Turkington , Michael Harding , Constanza Fleitas , and Randy
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4
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Kutcher . Cereal and Flax Pathology Group, Department of Plant Sciences, University of Saskatchewan,
College of Agriculture and Bioresources, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8;
2 Alberta Plant Health Lab, Crop Diversification Centre North, 17507 Fort Road NW, Edmonton, Alberta,
T5Y 6H3; Lacombe Research and Development Centre, Agriculture and Agri-Food Canada, 6000 C&E
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Trail, Lacombe, Alberta, T4L 1W1; and Alberta Agriculture and Irrigation, 301 Horticultural Stn Rd E,
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Brooks, Alberta, T1R 1E6
Correspondence to: randy.kutcher@usask.ca

Over the past decade, there has been a notable rise in outbreaks of bacterial leaf streak (BLS) in cereals
grown in many regions in Canada and the United States. This can be attributed to various factors,
including changes in agronomic practices, favourable weather conditions, and the absence of resistant
cereal cultivars. As a result, BLS, once considered to occur sporadically, has now become a prevalent
and damaging foliar disease in affected areas. Addressing the root causes of these outbreaks is
necessary to effectively manage the spread of BLS and mitigate its impact on cereal crop yield and
quality. Seed infection is the primary source of BLS inoculum; therefore, this project aims to detect the
bacterium on wheat kernels. We developed a Loop- mediated Amplification protocol (LAMP) to detect the
pathogen on seed as part of an integrated disease management strategy. The assay includes the design
of specific primers targeting a gene encoding a hypothetical protein specific to X. translucens pv.
undulosa, the pathovar that has the greatest effect on wheat. The LAMP assay amplifies the target DNA
rapidly under isothermal conditions, enabling simple and rapid visual detection and differentiation, without
sophisticated or specialized equipment. Specificity and sensitivity were tested using DNA of
Xanthomonas spp. and non-Xanthomonas spp. Of five specific primer sets, the most sensitive was
selected for the seed testing protocol. This protocol seeks to enhance our capacity to monitor BLS on
wheat samples, contributing to more effective disease control measures.

*[O187] EVALUATING THE INFLUENCE OF NITROGEN ON ROOT ARCHITECTURE AND
CLUBROOT RESPONSE IN BRASSICA GENOTYPES. Danna Rotariu, Yoann Aigu, Rudolph Fredua-
Agyeman, Sheau-Fang Hwang, and Stephen Strelkov. Department of Agricultural, Food and Nutritional
Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada
Correspondence to: Danna Rotariu; rotariu@ualberta.ca

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