Page 191 - Plant Canada 2024 Proceeding

PLANT CANADA 2024

exhibited marginal increases (erucic, oleic), one exhibited marginal decreases (linolenic), and two
exhibited no differences relative to conventional GS (linoleic, overall oil content).
The promising accuracy of GS observed in this study supports its potential utility in future Brassica
breeding programs.

[O171] CROSS-SPECIES COMPARATIVE SEQUENCE-BASED GENE EXPRESSION MODELLING IN
LEGUMES. Nicolas Raymond , Sheikh Jubair , Jordan Ubbens , Xi Zhang , Fatima Davelouis , Ruchika
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Verma , David Staszak , Dustin Cram , Halim Song , Yongguo Cao , Christine Sidebottom , Yasmina
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Bekkaoui , Morgan Kirzinger , Deborah Akaniru , and David Konkin . Alberta Machine Intelligence
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Institute, Edmonton, Canada; and Aquatic and Crop Resource Development, National Research Council
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of Canada, Saskatoon, Canada
Correspondence to David.Konkin@nrc.ca

Advances in genomics, machine learning and gene editing present an opportunity to accelerate trait
discovery and breeding by incorporating base-level functional interpretation into breeding and research
strategies. Natural genetic diversity represents an extremely rich resource for modelling gene expression
but comes with the challenges associated with non-independence of variation due to evolutionary
processes. In order to build neural network-based gene expression models that predict transcription and
transcript stability directly from DNA sequence, we are leveraging cross-species differences in gene
expression from matched RNA-seq and PRO-seq datasets for reference lineages of a variety of cool
season legumes including, field pea (Pisum sativum), grass pea (Lathyrus sativus), faba bean (Vicia
faba), and barrel medic (Medicago truncatula). Here, we detail our progress on these models, and
highlight potential applications for variant interpretation and transcript association studies.

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[O173] HUMIC PRODUCTS: TO USE OR NOT TO USE IN YOUR FIELD. Linda Y. Gorim . Department
of Agricultural, Food and Nutritional Science Room 410 Agriculture/Forestry Centre, University of Alberta,
Edmonton, Alberta, Canada T6G 2P5
Correspondence to: gorim@ualberta.ca

Humic products are widely sold to Canadian producers as either soil amendments or biostimulants.
Several humic products are available in solid and liquid forms. In Alberta, huge deposits of humalite have
been applied by producers but questions on appropriate humalite application rates for specific crops, its
impact of crop yield and soil health; and its interaction with nitrogen rates are unknown. Most trials on
humic products have been conducted indoors. Information from prairie-specific field trials to aid
producers’ decision making are lacking. Therefore, a study was initiated in 2021 at three sites
representing three soil zones with the objective to (i) identify humalite application rates for different soil
zones, and (ii) assess the effects of humalite in the presence of reduced urea on crop yield. We further
conducted an incubation experiment using soil from different soil zones to assess the interaction of soil
pH, humalite and soil nutrients. Results indicate that field application of humalite at 200 and 400 lbs/ac
produced high crop yields depending on urea rates. Soil with high SOM did not respond to humalite
application especially in a wet year. There are indications that humalite effects maybe moisture and crop
specific: More effects in wheat than canola and in dry years. Leaf applied humic product are not effective
– should be soil applied. Humic products differ and decision to use them should be economics based. A
strong interaction between humalite, soil zone and pH was observed in the incubation experiment.

[O174] GROWTH-PROMOTING RHIZOBACTERIA MITIGATES SALT STRESS IN RICE THROUGH
THE ENHANCEMENT OF ANTIOXIDANT DEFENSE, ION HOMEOSTASIS, AND PHOTOSYNTHETIC
PARAMETERS. Ayesha Siddika , Alfi Anjum Rashid , Shakila Nargis Khan , Amena Khatun ,
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Muhammad Manjurul Karim , PV Vara Prasad , and Mirza Hasanuzzaman . Department of Agronomy,
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Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh; Department of
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Microbiology, University of Dhaka, Dhaka 1000, Bangladesh; Noakhali Science and Technology
University, Noakhali-3814, Bangladesh; and Department of Agronomy, Kansas State University,
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Manhattan, KS, United States
Correspondence to: mhzsauag@yahoo.com

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