Page 144 - Plant Canada 2024 Proceeding

PLANT CANADA 2024

*[O85] PROTEOMIC ANALYSIS REVEALS NEW INSIGHTS RELATED TO THE INTERACTION
BETWEEN XANTHOMONAS PHASEOLI PV PHASEOLI AND PHASEOLUS VULGARIS L. Mylene
Corzo-Lopez , Jason McAlister , Boyan Liu , Jennifer Geddes-McAlister , and K. Peter Pauls . Plant
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Agriculture Department, University of Guelph, Guelph, ON, Canada N1G 2W1; and Molecular and
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Cellular Biology Department, University of Guelph, Guelph, ON, Canada N1G 2W1
Correspondence to: corzolom@uoguelph.ca

Xanthomonas phaseoli pv phaseoli (Xpp) is one of the causal agents of common bacterial blight (CBB)
disease in Phaseolus vulgaris L (common bean). As a strategy to invade the host, this pathogen injects
proteins into the plant cell that allow it to survive and multiply within the plant tissue and colonize the host.
The plant response to the invading organism depends on which plant-microorganism recognition and
immunity pathway is triggered, such as the Pathogen-Associated Molecular Pattern-Triggered Immunity
(PAMP-PTI) or the Effector-Triggered Immunity (ETI). PAMP-PTI pathways activate the expression of
genes associated with defense through Mitogen-Activated Protein Kinase (MAPK) cascades activated by
the production of reactive oxygen species. In addition, ETI occurs in plants that carry resistant proteins
that recognize the pathogen’s virulence effector proteins. To understand the molecular mechanisms
during the earliest stages of the plant pathogen interactions, a comparative proteomics analysis was
conducted of the infection of common bean genotypes with Xpp strains. Our research employed a
bottom-up proteomics approach to assess and characterize the interactions between two common bean
recombinant inbred lines (RIL), one CBB resistant and another CBB susceptible, with a pathogenic strain
and a non-pathogenic strain of bacteria. In this study, the Xpp differentially abundant proteins (DAPs)
associated with pathogen virulence included the outer membrane protein and the polyphosphate-
selective porin with higher abundance in the incompatible interaction at 48 hours post inoculation (hpi).
The CBB resistant RIL-DAPs after challenging with the pathogenic strain, were mainly involved in the
biosynthesis in secondary metabolites, such as isoflavones. Additionally, some DAPs were related to
plant-pathogen interactions, such as ubiquitin-mediated proteolysis, and glyoxylate and dicarboxylate
metabolism, demonstrating higher accumulation at 48 hpi. In contrast, the DAPs at 48 hpi after challenge
with the non-pathogenic strain were mainly related to oxidative phosphorylation, photosynthetic
processes, and pentose phosphate pathways. Interestingly, the CBB susceptible RIL showed no
discernible differences in protein expression after being challenged with the two bacterial strains at 48
hpi. However, proteins involved in starch and sucrose metabolism, linoleic acid metabolism, lipid
metabolism, vitamin metabolism, RNA degradation and glycolysis/glucogenesis pathways were induced
faster in the compatible interaction than in the incompatible interaction at 72 hpi. Overall, this study
provides valuable insights into the molecular mechanisms incompatible and incompatible interactions
between Xpp and P. vulgaris. These findings will contribute to the development effective strategies for
managing the common bacterial blight disease in common bean crop.

*[O86] INSIGHTS FROM NEXT GENERATION SEQUENCING: NOVEL VIRUSES AND VARIANTS IN
HIGHBUSH BLUEBERRIES OF BRITISH COLUMBIA. Sachithrani Kannangara , Juan Rodriguez ,
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Adam Gilewski , Gerda de Villiers , Megan Ellis , Peter Ellis , Eric Erbrandt , and Jim Mattsson .
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1 Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A
1S6, Canada; Phyto Diagnostics Company Ltd., 9381, Ardmore Drive, North Saanich, BC V8L 5G4,
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Canada; and BC Blueberry Council, 275 32160 South Fraser Way, Abbotsford, BC V2T 1W5, Canada
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Correspondence to: jim_mattsson@sfu.ca

Highbush Blueberry (Vaccinium corymbosum L.) is an important fruit crop in British Columbia. Viral
diseases are known to reduce the production of blueberries and are challenging to control. New viruses
have been identified in many crops with the development of Next-generation sequencing (NGS) without
relying on visual symptoms. This gives the advantage of detecting putative pathogens ahead of time and
developing detection methods. The main objective of this research is to gain an unbiased overview of
viruses in blueberry plants with virus-like symptoms using RNA-seq and develop more reliable diagnostic
methods for the precise detection of disease. RNA-seq libraries were made from rRNA-depleted RNA
extracts of 107 blueberry leaf samples with Scorch/Shock-like symptoms and 10 healthy samples. We
screened de-novo assembled sequences against the NCBI database to find assemblies with viral origin.
Results showed the presence of novel blueberry Scorch virus (BlScV) variants, two novel blueberry
Luteoviruses, and a Tombusvirus-like associated RNA (tlaRNA). Phylogenetic analysis of coat protein
(CP) divided BlScV variants into two main clusters. Further, we observed that the 5’ end of the BlScV
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