Page 294 - PC2019 Program & Proceedings

PLANT CANADA 2019

P161. The Canadian Collection of Fungal Cultures: What we have for you and what you have for us.
Robleh Djama, Z.; C. Robidas; B. Goulet; T. Rintoul
Agriculture and Agri-Food Canada

Preserving representatives of fungi can help farmers devise effective anti-fungal treatments for
agriculture, add to the growing body of knowledge of mycotoxins, identify invasive organisms or
beneficial fungi that could enhance agricultural or economic productivity. The Canadian Collection of
Fungal Cultures (DAOMC), in Ottawa, ON, is an internationally recognized culture collection
maintaining ~20,000 living cultures, representing more than 4,000 species of fungi. This poster will
present a synopsis of the diversity of the accessioned lineages within the collection. Partner institutions
submit and requested fungal isolates in support of research in a variety of research fields. These include:
zoosporic fungi, mycotoxigenic fungi, Aspergillius sp., Penicillium sp., Fusarium sp., Basidiomycota
obtained from Canadian agricultural environments, and members of the families Clavicipitaceae ,
Erysiphales and Dothideomycetes. We house a variety of known plant pathogenic fungi including:
Claviceps purpurea Ergot on rye, Pythium sp. and Phytophthora sp. the causal agent of damping off and
root rot, Fusarium head blight in wheat, barley and other grains caused by Fusarium sp., and Tilletia sp.,
the causal agent of bunts and smuts in cereals.

It is the hope and mandate of the Canadian Collection of Fungal Cultures to receive, store, preserve and
share fungal organisms in collaboration with the scientific community. Researchers are invited to contact
the CCFC to acquire or deposit fungal cultures.

Zeinab Robleh Djama (zeinab.roblehdjama@canada.ca)

TOPIC 21: Pathology, Epidemiology and Disease Management

(Posters P162-P196)

P162. Buckwheat rhizosphere as a host for unique bacterial species
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1
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Fofana, B. ; A. Alkhnajari ; K. Ghose ; A. Somalraju
1 Charlottetown research and development centre
2
Texas Tech University

Wireworm has become a major problem causing extensive crop loss in many potato production areas in
Canada. Control measures include the use of chemical pesticides. However, pesticides can affect human
health and the environment, and their use has consequently been limited in many countries. Alternative
control measures rely on integrated pest management including crop rotation. Hence, buckwheat is used
as a rotation crop to mitigate wireworm damage in potatoes. However, less is known about how
buckwheat contributes to mitigating the pest incidence. A 16S rRNA metagenomic study was conducted
to determine the microbiome associated with buckwheat in comparison with barley grown in two
locations with contrasting wireworm densities. The study identified 27 phyla associated with the
rhizosphere of the two crops and Proteobacteira, Bacteroidetes, Actinobacteria were found to be the most
abundant. Three non-pathogenic endophytic species, Methylophilus flavus, Saccharopolyspora tripterygii
and Deinococcus yunweiensis belonging to the Proteobacteira, Actinobacteria and Deinococcus-
Thermus phyla were found to be unique to the buckwheat rhizosphere soil from the two locations. The
data will be presented and discussed in the context of sustainable agriculture and rhizosphere microbiome
reformatting by rotation crops.

Bourlaye Fofana (bourlaye.fofana@canada.ca)

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